Single-cell full-length transcriptome sequencing (RNA-seq) is a technology that uses the 10x Genomics Chromium (10x) platform to obtain single-cell cDNA and combine it with transcriptome analysis on a long-sequence sequencing platform. This method combines the advantages of full-length transcriptome and single-cell sequencing, providing a powerful tool for discovering and quantifying isoforms, analyzing alternative splicing at the single-cell level.
Single-cell RNA sequencing (scRNA-seq) has made up for the limitations of bulk RNA sequencing (RNA-seq). This method can resolve gene expression profiles in single cells for more accurate transcriptome assessment, and is currently receiving widespread attention and application. There are two main characteristics of the single-cell experiments, including the barcoding of cDNA synthesized by each cell with cell-specific barcodes and of individual mRNA molecules with unique molecular identifiers (UMIs). Among them, the former method allows better quantification of transcripts at the single-cell level. Since UMIs are usually degraded, it requires the high accuracy of the sequencing platform. ScRNA-seq is now a widely adopted approach for profiling transcriptomic heterogeneity. However, assessing transcript-level changes between cell types using current scRNA-seq protocols remains challenging, as they rely on short-read sequencing. Nowadays, long-read sequencing technology is widely used in scRNA-seq to generate complete transcript information in a single cell, overcoming the fundamental limitations of short-read sequencing.
At CD Genomics, we offer two strategies for our customers to choose from, including Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) single-cell full-length transcriptome sequencing. Both methods have their own advantages in the field of RNA-Seq. Based on advanced platforms and years of experience in transcriptome analysis, we are committed to providing the best strategy to meet the project needs of our global clients. Our single-cell full-length transcriptome sequencing service has many applications. In addition to normal developmental and physiological studies, we focus on disease studies, such as tumor research and viral infections. If you have additional requirements or questions about our services, please feel free to contact us.