At a glance:
The success of SMRTbell library sequencing relies on the quality of the starting material. Factors such as DNA damage, carry-over contaminants, and improper handling can significantly impact sequencing performance, leading to lower yields, shorter read lengths, and compromised data quality. This article provides a comprehensive and in-depth analysis of troubleshooting strategies to address the quality of starting material, along with detailed recommendations to optimize SMRTbell library sequencing performance.
PacBio SMRTbell template preparation workflow. (Kong et al., 2017)
Understanding the characteristics of the fragment size distribution is essential for optimizing sequencing performance:
For research purposes only, not intended for personal diagnosis, clinical testing, or health assessment