At a glance:
Since its introduction, Pacbio SMRT sequencing technology has gained attention for its advantages of long reads (10-25k), single molecules, and no GC preference. In 2019, PacBio launched HiFi sequencing, which increases single-molecule sequencing accuracy to 99.9% (Q33). And now, the next-generation, high-throughput Revio system, launched in 2022, breaks through the throughput limit once again. With advanced sequencing consumables, robotics, and powerful computational capabilities, Revio delivers accurate long reads and methylation information directly from the sequencer in every run. Unleash yourself to discover more with a complete view of the genome, epigenome, and transcriptome on the Revio System. The Revio System provides metagenomics researchers with faster, more cost-effective long-read sequencing, resulting in higher-quality and more complete microbial datasets and access to new areas of discovery.
Revio is a gene sequencing system for human genetic analysis, cancer research, agricultural genomics, etc. The Revio System supports HiFi sequencing. It provides the foundation for long read, superior accuracy, and direct methylation detection, adding affordability, high throughput, and ease of use.
The consumables and software used with the Revio System are flexible and easy to use, greatly simplifying the steps and optimizing the experience. Whether you're in a genome center or a small lab, you'll experience it all:
Fast and reliable library preparation
Library construction for the Revio System supports manual or automated preparation using the SMRTbell Preparation Kit 3.0.
Fast run setup
Sequencing plates and run designs are automatically connected via an NFC tag, allowing run setup on the instrument in less than a minute.
Highly adaptable to your experimental schedule
The Revio system works independently of the four microarray benches, so the system can be loaded with consumables for up to 20 hours per day, ensuring full utilization of each microarray bench.
Built with multiple powerful NVIDIA GPUs, the Revio System delivers up to 20 times the computational power of the Sequel IIe System, delivering instantly available and accurate data directly from the instrument.
HiFi read generation
GPUs reduce turnaround time for base detection and HiFi read generation, outputting HiFi reads directly in BAM format.
Google Health DeepConsensus
DeepConsensus uses advanced deep learning techniques to further improve the accuracy and throughput of HiFi sequencing.
Standard runs include DNA methylation status, calculated using deep learning algorithms capable of handling polymerase kinetics.
All basic processing steps, including barcode splitting, are done on the instrument.
|Preserve the benefits of HiFi sequencing||Get more information on the Revio system|
|- Provides accurate long read of natural DNA molecules
- Use of Nanobind® CBB kits and SMR
- CBB kit and SMRTbell® preparation kit 3.0.
- Easy sample and library preparation
- Generate HiFi reads + methylation detection, barcode splitting on the instrument
- Standard BAM file format compatible with downstream analysis tools
|- High throughput - up to 1,300 HiFi genomes per year
- Onboard Google DeepConsensus for the most accurate HiFi reads available
- Simpler user experience with fewer consumables for fast runs
- 50% smaller output files for reduced storage costs
The Revio System excels at whole-genome sequencing, providing de novo assembly and variant detection. Its ability to sequence tens of bases in HiFi reads allows researchers to easily resolve large variants and navigate challenging genomic regions.
For more specific research requirements, the Revio system offers targeted sequencing. Whether it's sequencing amplicons that can process more than 1,000 samples in a single run, or targeted enrichment across the 20 Mb to 100 kb range, Revio meets a wide range of research needs.
The Revio system brings significant advantages to metagenomic research. Whether analyzing 384 communities through birdshot metagenomics or assembling data from 12 communities, the depth, and accuracy of the Revio system bring clarity to complex microbial datasets.
For transcriptomics, the Revio System provides single-cell transcriptomics tools that ensure comprehensive data of over 80 million reads per library.
For research purposes only, not intended for personal diagnosis, clinical testing, or health assessment