PacBio Pre-Made Library Sequencing

Customers can submit their libraries for full QC test and use our sequencing-only service. We are committed to providing state-of-the-art PacBio SMRT sequencing services with different capabilities and read lengths to meet the specific needs of our global customers.

PacBio SMRT Sequencing Platform at CD Genomics

Our platform consists mainly of third-generation PacBio sequencers, including Sequel II and Sequel IIe systems. Compared to Sequel I, these newer systems can generate up to 8x more sequencing data per SMRT-cell. Thanks to PacBio's latest chemistry (using longer-lifespan polymerases) and sequencing by cyclic consistent sequencing (CCS) analysis (with hairpin structured adapters), the Sequel II system is capable of generating High-Fidelity (HiFi) reads. In addition, PacBio sequencing chemistry is not sensitive to extreme GC contents compared to other sequencing technologies, so this technology can be applied to sequence regions with extreme GC contents.

Table 1. Data type, length, accuracy, and throughput across PacBio SMRT sequencing platforms. (Logsdon, G. A., et al., 2020)

Sequencing technology	Platform	Data type	Read length (kb)		Read accuracy (%)	Throughput per flow cell (Gb)
			N50	Maximum		Mean	Maximum
Pacific Biosciences (PacBio)	Sequel	Continuous long read (CLR)	25-50	>100		5–10	20
	Sequel II	Continuous long read (CLR)	30–60	>200		50–100	160
		HiFi read	10–20	>20	>99	15–30	35

Sample Requirements

1. Genomic DNA samples, >5µg, OD260/280=1.8~2.0, OD260/230=2.0~2.2, no degradation and no contamination
2. RNA samples, >5µg, OD260/280=1.8~2.1, OD260/230>1.5, no degradation and no contamination
3. We can handle the following list of samples:
   -Large-insert gDNA (20kb+)
   -HiFi template preparation (12-20kb)
   -BAC-based DNA
   -Purified PCR products
   -Multiplex amplicons.
   -Multiplex small genomes (microbes).

Application of Our Service

• Whole genome sequencing for de novo assembly
  Enable detection of undetected SNPs, regulatory regions embedded in complex structures, and fully intact genes.
• Whole-genome sequencing for structural variant calling
  SMRT sequencing can identify structural variants (SV) and Insertion–deletion mutation (inDel) by low-coverage, long-read whole-genome sequencing, and more than 80% of these variants are currently undetectable using standard short-read sequencing methods.
• RNA sequencing
  Transcriptome and isoform sequencing with PacBio's Iso-Seq protocol includes cDNA generation from total RNA and SMRTbell addition. PacBio sequencing can be applied to detect very long polycistronic RNA molecules, and novel transcriptional overlaps between adjacent and distant genes situated in parallel.

In addition, with the SMRT technology of the PacBio Sequel II system, we also offer a variety of services such as target sequencing, complex populations, and epigenetics. Just let us know your research needs. We will provide the best strategy to meet your research goals. Please don't hesitate to contact us for more details!