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Customers can submit their libraries for full QC test and use our sequencing-only service. We are committed to providing state-of-the-art PacBio SMRT sequencing services with different capabilities and read lengths to meet the specific needs of our global customers.
Our platform consists mainly of third-generation PacBio sequencers, including Sequel II and Sequel IIe systems. Compared to Sequel I, these newer systems can generate up to 8x more sequencing data per SMRT-cell. Thanks to PacBio's latest chemistry (using longer-lifespan polymerases) and sequencing by cyclic consistent sequencing (CCS) analysis (with hairpin structured adapters), the Sequel II system is capable of generating High-Fidelity (HiFi) reads. In addition, PacBio sequencing chemistry is not sensitive to extreme GC contents compared to other sequencing technologies, so this technology can be applied to sequence regions with extreme GC contents.
Table 1. Data type, length, accuracy, and throughput across PacBio SMRT sequencing platforms. (Logsdon, G. A., et al., 2020)
|Sequencing technology||Platform||Data type||Read length (kb)||Read accuracy (%)||Throughput per flow cell (Gb)|
|Pacific Biosciences (PacBio)||Sequel||Continuous long read (CLR)||25-50||>100||5–10||20|
|Sequel II||Continuous long read (CLR)||30–60||>200||50–100||160|
In addition, with the SMRT technology of the PacBio Sequel II system, we also offer a variety of services such as target sequencing, complex populations, and epigenetics. Just let us know your research needs. We will provide the best strategy to meet your research goals. Please don't hesitate to contact us for more details!