The Oxford Nanopore Technologies (ONT) sequencing system uses a novel nanopore technology for sequencing long stretches of DNA or RNA. Unlike NGS methods that detect secondary signals, Oxford Nanopore sequencing technology directly identifies the current changes generated in real-time during the process. Changes in ionic currents are different for each nucleotide, producing a unique signature for each base. Then the electrical signal is translated into nucleotide bases (i.e. base-calling). ONT sequencing supports hundreds of kb of read length, greatly enhanced de novo genome assemblies, structural genomic variant, and transcriptome studies.
We are providing Nanopore sequencing services that are scalable from miniature devices to high-throughput installations. Different types of Nanopore sequencing are available, including,
-DNA sequencing, including long-read and ultra-long-read DNA sequencing.
-Full-length cDNA sequencing.
-Direct RNA sequencing.
With the introduction of the Nanopore sequencing system, we have built a robust, scalable platform consisting of the MinION, GridION, and PromethION systems. These systems can now produce two major types of long reads with different lengths, accuracies, and throughputs, and can be used for specific applications.
Table 1. Data type, length, accuracy, and throughput across Oxford Nanopore sequencing platforms. (Logsdon, G. A., et al., 2020)
|Sequencing technology||Platform||Data type||Read length (kb)||Read accuracy (%)||Throughput per flow cell (Gb)|
|Oxford Nanopore Technologies (ONT)||MinION/GridION||Long read||10–60||>1,000||87–98||2-20||30|
Nanopore sequencing with ONT systems can yield huge read lengths due to its unique pore chemistry, which allows molecules to be transferred through the nanopore regardless of length. Here we outline the factors that affect the quality of ONT reads.
Fig2. ONT long and ultra-long reads. (Logsdon, G. A.,et al., 2020)
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