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Oxford nanopore RNA sequencing (RNA-seq) technology enables accurate analysis of full-length transcripts without the need to amplify or fragment, streamlining analysis and eliminating amplification bias and read length limitations. More importantly, Oxford Nanopore Technology (ONT) is the first long-read technique capable of directly sequencing native RNA strands. CD Genomics is now providing nanopore direct RNA ( direct cDNA or direct RNA)sequencing to achieve the ultimate goal of a comprehensive and bias-free understanding of transcriptomes. With years of experience in long-read RNA-seq analysis and advanced ONT platforms, we will be your best partner for direct RNA-seq.
To date, direct RNA-seq has been used in the study of various species, including Caenorhabditis elegans, Arabidopsis, yeast, and human cell lines. Compared with other sequencing technologies, nanopore direct RNA sequencing has its unique advantages, including,
-Direct RNA sequencing: RNA amount ≥1μg, RNA-integrity scores (RIN-scores) ≥ 8.
-Direct cDNA sequencing: RNA amount ≥ 1μg, RNA-integrity scores (RIN-scores) ≥ 8.
-The RNA samples need to be DNA-free and need to be accompanied by Bioanalyzer traces.
Different types of library preparation protocols can be used for direct cDNA/RNA sequencing. Real-time streaming of sequence data allows for fast insight into samples, on-demand sequencing, and dynamic workflows. Our service support,
-Classification of known and novel transcripts.
-Characterization of poly-A tail lengths of individual transcripts.
-Identification of allele-specific gene and complex RNA splice isoforms.
-Identification of RNA base modifications and RNA structure prediction.
-Quantify and study differential gene expressions.
-Characterization of RNA viruses and viral epidemiology.
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