We are dedicated to providing outstanding customer service and being reachable at all times.
CD Genomics is a leading provider of genomic services. We have extensive experience in long-read amplicon sequencing using the PacBio platform. PacBio SMRT long-read sequencing is capable of generating complete, uniform, unbiased coverage spanning long amplicons, allowing for a more realistic restoration of the microbial community structure in the samples.
16S/18S/ITS amplicon sequencing, also known as microbial diversity sequencing, refers to the sequencing of PCR products from specific regions such as 16S ribosomal DNA (rDNA), 18S rDNA, Internal Transcribed Spacer (ITS), and functional genes. This method does not require culturing microorganisms, and by detecting sequence variation and abundance in the target region, information on microbial community structure, evolutionary relationships, and microbial relevance to the environment can be obtained from environmental samples.
16S and 18S rDNA are the most commonly used marker genes in metataxonomics. Among them, 16S rRNA gene (~1,500bp) with nine variable regions interspersed between conserved regions, is used to analyze the diversity of bacteria or archaea. 18S rRNA gene (1,500-2,000bp) is utilized to reflect species differences in eukaryotes in a given sample. While ITS (400-900bp) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi, and archaea. ITS region has been widely utilized for the classification of fungal communities, including ITS1 and ITS2.
PacBio SMRT long-read sequencing has overcome the limitations of short reads, allowing researchers to obtain full-length reads of 16s rDNA in bacteria or 18S rDNA and ITS regions in eukaryote.
Standard Analysis | Advanced Analysis |
-OTU clustering and annotation -Species annotation -Species composition analysis -Alpha diversity analysis -Beta diversity analysis -Functional prediction -Multi statistics |
-Species variation analysis -Correlation analysis of environmental factors -Phylogenetic analysis -Customized analysis |
If you are interested in our full-length 16S/18S/ITS amplicon sequencing service, please feel free to contact us. We've got everything covered for your needs and are ready to assist.