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Epigenetic modifications refer to chemical changes in nucleic acids that do not alter DNA sequence but play a key role in genetics, growth, lifespan, aging, and disease, mainly including DNA methylation and histone modifications. With the explosion of technology and understanding in the last decade, researchers realized that RNA methylation is another dynamically regulated, reversible, and widespread post-transcriptional regulator associated with a variety of basic and disease-related biological processes. Using long-read sequencing technologies (PacBio SMRT and Oxford Nanopore sequencing), researchers can directly detect DNA and RNA methylation modifications, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), and N6-methyladenine (6mA) in DNA, and N6-methyladenosine modification (m6A), 5-methylcytosine modification (m5C), and N1-methyladenosine (m1A) in RNA. With years of experience in epigenetics and methylation analysis, our team of experts is dedicated to providing cost-and sequencing-effective solutions for DNA and RNA methylation detection and analysis in plants and animals.
Since both PacBio SMRT and Nanopore sequencing technologies sequence native unamplified templates, base modifications of DNA and RNA molecules can be preserved, allowing epigenomic changes to be studied by polymerase kinetics or current changes, respectively. Prior to the application of long-read sequencing, the combination of bisulfite treatment and short-read sequencing, known as bisulfite sequencing (BS-seq), served as a common and powerful tool for detecting base modifications (5mC in DNA). Compared to BS-seq methods, native DNA and RNA sequencing via PacBio and/or Nanopore sequencing technology possess a range of advantages, including,
CD Genomics is a leading global life sciences company. We specialize in the application of third-generation sequencing technologies, including PacBio SMRT and Oxford Nanopore sequencing. We have years of experience in long-read sequencing and are committed to providing effective and cost-effective contract research and developmental services to the global pharmaceutical, medical technology, crop protection, and chemical industries. If you are interested in our services, please don't hesitate to contact us. We are looking forward to cooperating with you.