We are dedicated to providing outstanding customer service and being reachable at all times.
CD Genomics is providing long-read sequencing technologies developed by Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio) to fully support the de novo assembly of bacterial genomes. To ensure maximum assembly accuracy, we recommend using short-read Illumina data to further polish the genome assembly. Our services are end-to-end and customized, enabling the rapid delivery of more cost-effective strategies and high-quality genome assemblies.
Short-read sequencing technologies, such as the Illumina platform, are now widely used in bacterial genome research due to its ability to provide rapid and accurate analysis of the entire bacterial genome. However, short-read sequencing, in addition to being limited by amplification and sequencing bias, has inherent limitations that generally do not allow for whole-genome assembly. Long-read sequencing technologies developed by ONT and PacBio can produce long reads in tens of kilobase pairs, thus overcoming the limitations of short-read sequencing to obtain a complete assembly. As the first established long-read technology, PacBio Single Molecular Real-Time (SMRT) sequencing is based on a sequencing-by-synthesis method. The PacBio sequencer can generate the average length of reads ranging from 10-14 kb and can reach throughputs of about 30 Gb per run. ONT long-read sequencing has no upper read length limit and can assemble the complete microbial genome relatively quickly and inexpensively, which has gained increasing popularity. This technique has been used to assist in the completion of the genome sequence of Staphylococcus aureus. With the advanced technologies and improved analysis pipelines, ONT long-read sequencing can be applied for complex bacteria, such as Fusobacterium nucleatum, Pseudomonas koreensis, Mycobacterium tuberculosis and other genomes with long repetitive sequences.
Sequencing Platform | Illumina HiSeq PE150 | PacBio Sequel ll | Nanopore PromethION |
Sample Requirements | Sample Type: genomic DNA Amount:≥ 200ng OD260/280=1.8~2.0 no degradation and no contamination |
Sample Type: HMW genomic DNA Amount:≥ 15μg OD260/280=1.7~2.0 Fragments should be ≥ 20 kb |
Sample Type: HMW genomic DNA Amount:≥ 10μg OD260/280=1.75~2.0 Fragments should be ≥ 20 kb |
Sequencing Strategies | 350 bp insert library, ≥ 50 X | 20Kb SMRTbell library, CLR sequencing, ≥100X | ONT Library preparation, ≥100X |
Application Scope | Large-scale sequencing and rapid scanning | Sequencing of a small number of strains Accuracy in place |
Sequencing of a small number of strains Accuracy in place |
Delivery Cycle | Minimum of 30 working days | Minimum of 45 working days | Minimum of 40 working days |
Analysis of bacterial genome information at different levels of fineness, deciphering the development of bacteria, and exploring their potential value. Our analysis consists of two main parts: standard analysis and advanced analysis. Specific analytical services are as follows, but are not limited to,
To perform bacterial whole-genome de novo sequencing, researchers choose CD Genomics. With multiple specialists and years of experience in this field, we guarantee you high-quality data and integrated bioinformatics analyses. Please feel free to contact us. We are happy to help you!
References
For research purposes only, not intended for personal diagnosis, clinical testing, or health assessment