Animal/Plant Whole Genome Sequencing

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Animal/Plant Whole Genome Sequencing

CD Genomics' animal and plant whole genome sequencing, also known as animal and plant whole genome resequencing (WGRS), namely sequencing the entire genome of a plant or animal and aligning it to a known reference genome. We have two long-read sequencing platforms, PacBio SMRT sequencing and ONT nanopore sequencing, which provide comprehensive access to coding and non-coding regions of chromosomal, mitochondrial DNA, and chloroplast (plant) DNA.

Overview of Animal and Plant Whole Genome Sequencing

View of a Businessman holding a DNA growing as a plant.

Animal and plant whole genome sequencing (WGS) is a powerful technique that is commonly used to achieve an accurate and comprehensive examination of the genomes of animals and plants. WGS methods offer many advantages over traditional methods such as genotyping sequencing and arrays, including the generation of orders of magnitude more data, relatively enhanced variant discovery, greater statistical power, and easier analysis. This technology can reveal the molecular mechanisms of breeding and species formation, identify common genetic variations among populations, and provide resources for accelerated genetic development. As a result, animal and plant whole genome sequencing has been used for a wide range of applications, including the study of the origin and evolution of species, genome-wide association studies (GWAS), and agricultural breeding projects.

Sample Requirements

  1. Genomic DNA amount:
  2. -PacBio Sequel II sequencing platform: HMW Genomic DNA≥ 15μg, OD260/280 =1.8 ~2.0, A260/230=1.5 ~2.6
    -Nanopore PromethION sequencing platform: HMW Genomic DNA≥ 8μg, OD260/280 =1.75 ~2.0, A260/230=1.4 ~2.6

  3. For additional sample inquiries, please contact our professional technicians.

Workflow of Our Animal/Plant Whole Genome Sequencing Services

CD Genome provides professional services from experiment consultation and design, library construction and quality control (QC), long-read sequencing to data analysis. Each step is carefully designed and follows high scientific standards to ensure the reliability and accuracy of study results.

Workflow of Our Animal Plant Whole Genome Sequencing Services-CD Genomics.

Analysis Pipeline and Contents

  • Raw data processing.
  • Standard analysis.
    -Aligning to the current reference genome.
    -Alignment statistics, including depth distribution and coverage calculation.
    -Genetic variation detection, annotation, and statistics.
  • Advanced analysis.
    -Comparative genome analysis.
    -Customized genome analysis.

Analysis Pipeline and Contents-CD Genomics.

Benefits of Our Services

-Decades of experience in genomics services and innovative technology platforms.
-Flexible sequencing strategies and customized bioinformatics pipelines.
-Supporting a broad range of species.
-Fast turnaround time and complete analytical study reports.
-Most competitive prices.

We have years of experience in providing accurate and customized whole genome sequencing services and high-quality results. Based on advanced long-read sequencing platforms, our service generates reference-quality assemblies of complex plant and animal genomes by overcoming problems such as high GC regions that cannot be spanned, highly repetitive fragments that cannot be accurately quantified, and rare mutations that are overwhelmed. In addition, almost all types of genetic variation, such as single nucleotide variants (SNV), small fragment insertion-deletion mutations (InDel), and large fragment structure variants (SV), can be detected and analyzed. Please feel free to share any comments, questions, or requests you might have. Our expert and experienced team will be in contact with you as soon as possible.

Reference

  1. Rehman, S. U., et al. (2021). "Whole-genome sequencing and characterization of buffalo genetic resources: recent advances and future challenges." Animals, 11(3), 904.
For Research Use Only. Not for use in diagnostic procedures.

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