Long sequence sequencing (LSR) technologies enable end-to-end sequencing of entire RNA or cDNA molecules, making them possible to sequence individual full-length transcripts with high precision. These advanced sequencing technologies have been widely applied in a variety of plants and animals, refining existing gene annotations and describing the prevalence of alternative splicing among organs. Based on Oxford Nanopore Technologies (ONT) nanopore sequencing and Pacific Biosciences's (PacBio's) Single Molecule, Real-Time (SMRT) sequencing, CD Genomics is providing animal and plant RNA sequencing and leading to breakthrough discoveries.
The goals of transcriptomics include transcriptome annotation, the determination of the functional structure of each gene in the genome, and measurement of the changes in the expression levels of each gene in different transcriptome samples. At present, next-generation sequencing remains the primary method used for transcriptome research. However, short reads (fragmented transcripts) cannot span the entirety of the transcripts, greatly limiting the recognition of complex transcript isoforms. In order to overcome the fundamental limitations of short-read RNA-seq, LSR technologies have been developed and used. To date, PacBio Iso-seq has been used to explore many full-length cDNA samples from plants and animals. In recent years, Oxford nanopore sequencing has also been utilized to sequence full-length cDNA samples from model animals (such as mice) and humans. In practical applications, each long-read sequencing has its own advantages and disadvantages.
Fig1. Different applications and bioinformatics solutions for PacBio Iso-seq and Nanopore direct RNA sequencing in plants. (Zhao, L., et al., 2019)
-Poly-A tail length estimation prediction.
-RNA base modification identification.
-Gene and isoform quantification.
-New transcript isoform annotation.
-Complex alternative splicing (AS) analysis.
-Alternative polyadenylation (APA) event identification.
-Novel RNA prediction.
-Fusion transcript analysis.
-Differential gene expression analysis.
CD Genomics is a leading custom service provider in the field of long-read sequencing. Our goal is to help clients accelerate their research and support their careers by using advanced third-generation sequencing platforms. By developing innovative, tailored, and cost-effective strategies, we can greatly accelerate the success of their projects. Here, we are proud to offer long-read RNA sequencing service packages using Oxford nanopore sequencing or PacBio Iso-seq analysis. If you are interested in our services, please don’t hesitate to contact us. We look forward to working with you on your next project.